| Solution Information | help | |
| Enzyme: | Glucose-6-phosphate 1-dehydrogenase | |
| inhibitor: | BDBM61954 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | G6PDH counter-screening assay protocol The basic screening procedure for the G6PDH HTS assay follows a stepwise addition of reaction mixture components (as follows): 1. 15 uL of a 30 uM concentration of test compound is added to appropriate wells (20 point concentration range - 20 uM highest concentration) 2. 15 uL of a G6P and NAD+ mixture is added for a final concentration of 0.2 mM and 0.6 mM respectively. 3. 15 uL of G6PDH enzyme is added per well (final concentration = 0.006 mUnits/uL). 4. Reaction incubates for 1 hour at room temperature. 5. 5 uL EDTA is added to each well (final concentration = 50 mM). This assay was used as a counterscreen for the TbHK1 small molecule inhibitor analogues generated by the University of Kansas. These compounds were also rescreened in the TbHK1 dose response assays. This data reported within this assay indicates as to whether the analogues synthesized interfere with the assay format. | |
| If you find an error in this entry please send us an E-mail | ||